Frequently Asked Questions

  • Novel platform accelerates discovery (weeks vs. years)
  • Screening full or partial proteomes consisting of hundreds or thousands of proteins at a time
  • Only microliters of serum needed for hybridization
  • Highly reproducible microarray manufacturing and serological results

A typical study involves well-characterized serum samples and ADi protein arrays (either readymade or custom). After hybridization, data acquisition, and statistical analysis, disease associated antigens are identified. The study can be done in two ways:

1) Customers purchase protein arrays and reagents from ADi and carry out the study in their own lab.

2) Customers provide serum samples to ADi, and ADi will perform hybridization, data acquisition, Expert Statistical Analysis, and preparation of final report.

A typical study involves a comparison between a control group and one or more experimental groups. For example, a group of healthy donors can be compared to a group of donors with a particular disease or disease state. Expert Statistical Analysis is used to compare proteome wide immune profiles and identify novel disease associated biomarkers.

The number of samples in each group depends on the biological variability of the immune response to your disease of interest. For example, a very homogeneous response to a virus may require tens of samples, whereas heterogeneous immune responses to bacteria or human disease may require hundreds to thousands.

Serum, plasma or whole blood.

The preferable method is frozen in dry ice, we have also used samples shipped on wet ice or dried on filter paper.

Depending on the study, as little as 10 microliters or up to 50 microliters.

We can identify all immunologically reactive proteins displayed on a given full or partial proteome chip.

We have experience detecting IgG, IgM and IgA.

Any study that would normally be performed using an assay where a protein attaches to a substrate.  For example: serological ELISA-like assays, protein-protein interaction assays, and kinase assays. Unlike traditional experiments, protein microarrays give you the ability to run hundreds or even thousands of parallel assays.

Fill out the study proposal form on the website. ADi will review your proposal and contact you to discuss the next steps. After approving the study, ADi will provide specimen shipping instructions. Raw data and initial Expert Statistical Analysis will be available 90 days after receiving your samples.

  • Raw data – Signal intensity values for each protein and each sample in a Microsoft Excel file.
  • Heatmap – A color-coded matrix in terms of signal intensity that allows for the simultaneous viewing of all the data.
  • Expert Statistical Analysis (ESA) – In over 116 studies, Antigen Discovery’s dedicated staff of statisticians has validated and tested statistical models and methods to identify protein biomarkers.

Yes, the heatmap and/or Expert Statistical Analysis (ESA) can be generated at any time after the study. The cost will be on your quote.

Once a study design is completed, the material cost of the study is calculated based on the type and number of arrays, and reagents required.  For a study involving full service, the cost depends on the number of specimens, number of analytes and type of data report requested. Exact pricing can be determined from the submitted study proposal form.

24 months if properly stored (room temperature with a desiccator)

ADi’s proprietary platform uses cutting edge protein microarray technology, which allows for thousands or even tens of thousands of parallel assays to detect specific antibodies present in serum or plasma. Our approach allows researchers to profile the humoral immune response to an entire proteome.

Yes, results obtained from microarray experiments are highly reproducible. We have tested the robustness of our microarray chips under a variety of conditions (e.g. various chip lots, reagent lots, operators, days and different detection reagents).  Our results consistently demonstrate reproducibility, with R-squared values >0.9.

We have created a platform that allows for the parallel assaying of hundreds or thousands of proteins.  Our ever-growing inventory encompasses more than 35,000 proteins from over 30 microorganisms. Our Expert Statistical Analysis has been developed over the last 6 years to accurately, efficiently and reliably generate results with tremendous reproducibility. The power of our technology is demonstrated in over 40 scientific papers published by ADi and our collaborators. Now our powerful proteomic technology is available to researchers and clinicians worldwide.

ADi ELISA and AntigenStrip kits are all developed using our full proteome microarray screening technology.  We thoroughly examine the full proteome in order to select the best antigens for translation to an ELISA.

There is free training available at ADi headquarters in Irvine, CA. You are responsible for your travel arrangements. Please contact us in advance to schedule your visit.

There is also an option to have ADi staff come to your facility for a training session. Please inquire about the fee.